Search results

This paper proposes to examine a simple and cost‐effective method of integrating a reflector surface with a silicon‐based microfluidic channel for enhanced biosensing through the method of fluorescence in a microfluidics and nanofluidics‐based lab‐on‐a‐chip device.

Herein, the reflector is integrated with silicon‐based microfluidic channels and fluorescence measurements were carried out using alexafluor 647 particles. Two types of microfluidic channel surfaces were used, with and without reflector integration, for the experiments.

The experimental results prove that the proposed technique of partial reflector integration within microfluidic or nanofluidic channel surfaces is highly suitable for fluorescence‐based detection of single molecules and low concentration fluorophore‐tagged receptors.

It is believed that this is a novel work of integrating a reflector with a microfluidic channel surface for fluorescence‐based biodetection. This method will be very useful for fluorescence‐based biosensors in detecting low concentration fluorophores and single molecules.

This study aims to introduce a vision-based model to generate droplets with auto-tuned parameters. The model can auto-adjust the inherent uncertainties and errors involved with the fabrication and operating parameters in microfluidic platform, attaining precise size and frequency of droplet generation.

The photolithography method is utilized to prepare the microfluidic devices used in this study, and various experiments are conducted at various flow-rate and viscosity ratios. Data for droplet shape is collected to train the artificial intelligence (AI) models.

Growth phase of droplets demonstrated a unique spring back effect in droplet size. The fully developed droplet sizes in the microchannel were modeled using least absolute shrinkage and selection operators (LASSO) regression model, Gaussian support vector machine (SVM), long short term memory (LSTM) and deep neural network models. Mean absolute percentage error (MAPE) of 0.05 and R² = 0.93 were obtained with a deep neural network model on untrained flow data. The shape parameters of the droplets are affected by several uncontrolled parameters. These parameters are instinctively captured in the model.

Experimental data set is generated for varying viscosity values and flow rates. The variation of flow rate of continuous phase is observed here instead of dispersed phase. An automated computation routine is developed to read the droplet shape parameters considering the transient growth phase of droplets. The droplet size data is used to build and compare various AI models for predicting droplet sizes. A predictive model is developed, which is ready for automated closed loop control of the droplet generation.

Microfluidic or “lab‐on‐a‐chip” technology is seen as a key enabler in the rapidly expanding market for medical point‐of‐care and other kinds of portable diagnostic device. The purpose of this paper is to discuss two proposed packaging processes for large‐scale manufacture of microfluidic systems.

In the first packaging process, polymer overmoulding of a microfluidic chip is used to form a fluidic manifold integrated with the device in a single step. The anticipated advantages of the proposed method of packaging are ease of assembly and low part count. The second process involves the use of low‐frequency induction heating (LFIH) for the sealing of polymer microfluidics. The method requires no chamber, and provides fast and selective heating to the interface to be joined.

Initial work with glass microfluidics demonstrates feasibility for overmoulding through two separate sealing principles. One uses the overmould as a physical support structure and providing sealing using a compliant ferrule. The other relies on adhesion between the material of the overmould and the microfluidic device to provide a seal. As regards LFIH work on selection and structuring of susceptor materials is reported, together with analysis of the dimensions of the heat‐affected zone. Acrylic plates are joined using a thin (<10 μm) nickel susceptor providing a fluid seal that withstands a pressure of 590 kPa.

Microfluidic chips have until now been produced in relatively small numbers. To scale‐up from laboratory systems to the production volumes required for mass markets, packaging methods need to be adapted to mass manufacture.

This study aims to use an additive process for the first time to develop a microfluidic device that uses centrifugal technique for precise and repeatable generation of microdroplets. Droplets have versatile applications in life sciences, but so far centrifugal devices for their production have been made mainly using standard subtractive techniques. This study focused on evaluating the applicability of 3D printing technology in the development of centrifugal microfluidic devices and investigating their properties and future applications.

First, the background of this interdisciplinary research, including the principle of droplet microfluidics and the centrifugal technique, is explained. The developed device has the form of a disc (similar to an audio CD), containing an integrated microfluidic system for droplet generation. The disc is rotated at a specific spin profile to induce controlled liquid flow and accurate production of oil-in-water microdroplets. The device was fabricated using material jetting technology. The design, operation principles, printing process parameters and post-processing steps are explained in detail.

The device was thoroughly characterised, including its mechanical properties, the impact of chemical treatment and the flow measurement of the liquids. The study confirms that the disc can be applied to produce various emulsions using centrifugal force alone. 3D printing technology enables potential mass production and other applications of the device.

The 3D printing process allowed for easy design, fabrication and duplication of the device. Compared to standard PMMA discs, a simpler fabrication protocol and a more flexible and monolithic structure were obtained. The device can be adapted to other microfluidic processes in a lab with high potential for point-of-care applications.

This paper aims to focus on the application of low temperature co-fired ceramic (LTCC) technology in the fabrication of a microfluidic module with integrated microwave components. The design, technology and performance of such an LTCC-based module is investigated. The rapid heating of liquid samples on a microliter scale is shown to be possible with the use of microwaves.

The developed microwave-microfluidic module was fabricated using well-known LTCC technology. The finite element method was used to design the geometry of the microwave circuit. Various numerical simulations for different liquids were performed. Finally, the performance of the real LTCC-based microwave-microfluidic module was examined experimentally.

LTCC materials and technology can be used in the fabrication of microfluidic modules which use microwaves in the heating of the liquid sample. LTCC technology permits the fabrication of matching circuits with appropriate geometry, whereas microwave power can be used to heat up the liquid samples on a microliter scale.

The main limitation of the presented work is found to be in conjunction with LTCC technology. The dimensions and shape of the deposited conductors (e.g. microstrip line, matching circuit) depend on the screen-printing process. A line with resolution lower than 75 µm with well-defined edges is difficult to obtain. This can have an effect on the high-frequency properties of the LTCC modules.

The presented LTCC-based microfluidic module with integrated microwave circuits provides an opportunity for the further development of various micro-total analysis systems or lab-on-chips in which the rapid heating of liquid samples in low volumes is needed (e.g. miniature real-time polymerase chain reaction thermocycler).

Examples of the application of LTCC technology in the fabrication of microwave circuits and microfluidic systems can be found in the available literature. However, the LTCC-based module which combines microwave and microfluidic components has yet to have been reported. The preliminary work on the design, fabrication and properties of the LTCC microfluidic module with integrated microwave components is presented in this paper.

The purpose of this paper is to focus on the technology and performance of the miniature microfluidic module for urea determination. The presented module was made using low-temperature co-fired ceramics (LTCC). It shows the possibility for the integration of the bioreceptor layers with structures that have been fabricated using modern microelectronic technology.

The presented microfluidic module was fabricated using LTCC technology. The possibility for the fabrication of an enzymatic microreactor in a multilayer ceramic substrate, made of CeramTec glass ceramic (GC) material systems with an integrated thick-film heater, is studied. Different configurations of the LTCC/heater materials (gold, silvers and palladium-silver) are taken into account. The performance of the LTCC-based microfluidic module with the integrated heater and immobilized enzyme was examined experimentally.

A compatible material for the heater embedded in the CeramTec GC-based structures was found. The preliminary measurements made for the test solution containing various concentrations of urea have shown stability (for seven days of operation) and a relatively high signal-to-noise ratio (above 3 pH units) for the microreactor’s output signal.

The presented research is a preliminary work which is focused on the fabrication of the LTCC-based microfluidic module, with an integrated heater and immobilized enzyme for urea determination. The device was positively tested using a model reaction of the hydrolysis of urea. However, urea concentration in real (biological) fluid should also be measured.

The development of the LTCC-based microfluidic module for urea determination provides opportunity for the construction of a lab-on-chip, or μTAS-type system, for fast medical diagnoses and the continuous monitoring of various biochemical parameters, e.g. for estimating the effectiveness of hemodialysis.

This paper shows the design, fabrication and performance of the novel microfluidic module for urea determination, made with LTCC technology.

This paper aims to present a research on utilization of an irreversible bonding between non-transparent low temperature co-fired ceramics (LTCC) and transparent poly(dimethylsiloxane) (PDMS). The research presented in this paper is focused on the technology and performance of the miniature microfluidic module for fluorescence measurement.

The chemical combination of both materials is achieved through surface modification using argon-oxygen dielectric barrier discharge (DBD) plasma. According to the performed spectroscopic analyses (X-ray photoelectron spectroscopy, XPS; attenuated total reflection-Fourier infrared spectroscopy, ATR-FTIR) and contact angle measurements, the LTCC and PDMS surfaces are oxidized during the process. The presented microfluidic module was fabricated using LTCC technology. The possibility for the fabrication of LTCC-PDMS microfluidic fluorescent sensor is studied. The performance of the sensor was examined experimentally.

As a result of DBD plasma oxidation, the LTCC and PDMS surfaces change in character from hydrophobic to hydrophilic and were permanently bonded. The presented LTCC-PDMS bonding technique was used to fabricate a microfluidic fluorescent sensor. The preliminary measurements of the sensor have proven that it is possible to observe the fluorescence of a liquid sample from a very small volume.

The presented research is a preliminary work which is focused on the fabrication of the LTCC-PDMS fluorescent sensor. The microfluidic device was positively tested only for ethanolic fluorescein solutions. Therefore, fluorescence measurements should be performed for biological specimen (e.g. DNA).

The LTCC-PDMS bonding technology combines the advantages of both materials. One the one hand, transparent PDMS with precise, transparent three-dimensional structures can be fabricated using hot embossing, soft lithography or laser ablation. On the other hand, rigid LTCC substrate consisting of microfluidic structures, electric interconnections, heaters and optoelectronic components can be fabricated. The development of the LTCC-PDMS microfluidic modules provides opportunity for the construction of a lab-on-chip, or micro-total analysis systems-type system, for analytical chemistry and fast medical diagnoses.

This paper shows utilization of the PDMS-LTCC bonding technology for microfluidics. Moreover, the design, fabrication and performance of the PDMS-LTCC fluorescent sensor are presented.

This study aims to describe the effect of ironing process parameters on mixing efficiency and gradient generation in Y-micromixers and microfluidic gradient generators (MGGs), respectively.

Material extrusion (MEX) enables the production of miniaturized devices with the advantage of lower manufacturing costs and higher design freedom. However, surface finishing is the most important drawback when it comes to microfluidic applications where flow splitting is not required. First, the effect of ironing line spacing (LS) and speed (IS) on mixing efficiency in Y-micromixers was experimentally investigated. Then, the best ironing settings were chosen to further study the spatial stability of the normalized concentration gradient in MGGs.

Lower ironing LS and IS enhance the microchannel surface smoothness. The best combination of ironing parameters (lowest values of LS and IS) leads to an increase in mixing length of 191% at Q = 10 µL/min and 198% at Q = 20 µL/min, with respect to a similar Y-micromixer geometry where ironing was not performed. These findings were applied in the production of a MGG, showing that the normalized concentration gradient in the crosswise flow direction does not depend on the streamwise position when ironing is performed.

To the best of the authors’ knowledge, for the first time, the possibility of optimizing ironing parameters to enhance the surface roughness in MEX microfluidic devices has been investigated. Ironing of the channel bottom surface allows to reduce ridges-induced flow convection, thus delaying mixing in Y-micromixers and achieving stable concentration gradient in MGGs.

The purpose of this study is to design, fabricate and investigate low-temperature co-fired ceramic (LTCC) structures with integrated microfluidic elements. Special attention is paid to the study of fluid properties of micro-channels and microvalves, which are important constitutive parts of both, microfluidic systems and individual microfluidic devices.

Several test patterns of fluid channels with different geometry and different types of valves were designed and realized in LTCC technology. All test structures were tested under the flow of two fluids (liquids): water and isopropyl alcohol. Flow rates at different applied pressure were measured and hydrodynamic resistance and diode effect were calculated.

The investigation of the channels showed that viscosity of fluidic media has significant influence on the hydrodynamic resistance in channels with rectangular cross-section, while this effect is small on channels with square cross-section. The viscosity also has a decisive influence on the diode effect of different shape of valves, and therefore, it is important in the selection of the valve in practical applications.

In this work, the investigation of hydrodynamic resistance of channels and diode effect of passive valves is limited on selected geometry and only on two fluidic media and two applied pressures. All these and some other parameters have a significant influence on fluidic properties, but this will be the topic of the next research work, which will be supported by numerical modelling.

The presented results are useful in the future designing process of LTCC-based microfluidic devices and systems.

Microfluidic in the LTCC structures is an unconventional use of this technology. Therefore, the fluid properties are relatively unsearched. On the other hand, the global use of microfluidic devices and systems is growing rapidly in various applications. They are mostly made by polymer materials, however, in more demanding applications; ceramic is a useful alternative.

Biotechnology is closely associated to microfluidics. During the last decade, designs of microfluidic devices such as geometries and scales have been modified and improved according to the applications for better performance. Numerous sensor technologies existing in the industry has potential use for clinical applications. Fabrication techniques of microfluidics initially rooted from the electromechanical systems (EMS) technology.

In this review, we emphasized on the most available manufacture approaches to fabricate microchannels, their applications and the properties which make them unique components in biological studies.

Major fundamental and technological advances demonstrate the enhancing of capabilities and improving the reliability of biosensors based on microfluidic. Several researchers have been reported verity of methods to fabricate different devices based on EMS technology due to the electroconductivity properties and their small size of them. Therefore, controlled fabrication method of MEMS plays an important role to design and fabricate a highly selective detection of medical devices in a variety of biological fluids. Stable, tight and reliable monitoring devices for biological components still remains a massive challenge and several studies focused on MEMS to fabricate simple and easy monitoring devices.

This paper is not submitted or under review in any other journal.