A simple and efficient method for separation and purification of troxerutin on the SZ-3 resin

Abstract

Purpose

In view of the current difficulty of separation of troxerutin, the purpose of the paper is to separate and purify semi-synthetic flavonoid compound troxerutin by macroporous adsorption resin (SZ-3).

Design/methodology/approach

Comparing the adsorption performance and resolution of three different polar resins and choosing a resin to optimize the process parameters such as sample volume, eluent concentration and elution temperature to obtain high-purity troxerutin. After separating and enriching by resin column chromatography, detected the sample by LC-MS analysis.

Findings

This research found that the optimal conditions of the adsorption and desorption were sample volume S = 90 mg/g resin, methanol concentration C = 25%, T = 20 °C. the content of troxerutin increased significantly from 88% to more than 96%. Then confirmed the sample was troxerutin by LC-MS. In addition, the resin could be used for at least 10 cycles in the separation and purification experiments of troxerutin.

Originality/value

Purification of troxerutin with new SZ-3 resin for the first time. Under the optimal conditions, the purity and recovery of troxerutin was 96.4% and 39%. In this study, the authors established a purification process of troxerutin successfully that was simple, economical, environment friendly, with high purity and high recovery rate to provide a reference program for changing the status of troxerutin separation difficulties.

Citation

Li, Y., Mu, X., Kong, H., Pan, H. and Liu, H. (2021), "A simple and efficient method for separation and purification of troxerutin on the SZ-3 resin", Pigment & Resin Technology, Vol. 50 No. 4, pp. 338-345. https://doi.org/10.1108/PRT-08-2020-0083