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This paper aims to evaluate the separation and purification characteristics of flavonoids from polygonum cuspidatum (PC) extracts by using macroporous adsorption resins (MAR) mixed bed to improve the utilization rate of flavonoids.

Taking the separation performance of flavonoids as an evaluation index, the best MAR were screened from 31 sorts of MAR and combined the best MAR to form a MAR mixed bed for adsorption and separation of flavonoids.

By studying the separation conditions that affect flavonoids, the results showed that resin LZ-72 has best separation and purification effect on flavonoids under the optimal adsorption and desorption conditions, the purity of the obtained flavonoid compound reaches 82.50%, 2.66 times of the initial extract, and the recovery rate reaches 89.70%. Theoretical research results have shown that the adsorption of flavonoids by MAR conforms to the pseudo-second-order kinetics and Freundlich models.

Because the flavonoids in PC have great medicinal value, the purpose of this work is to develop a method of separating and purifying flavonoids from PC, which will provide a certain foundation for the development of medicine.

This contribution provided a novel way to separate flavonoids from PC. Under the optimal conditions, the content of flavonoids in the product was increased 2.66-fold from 31.01% to 82.50%, and the recovery yield was 89.70%.

In this study, a novel glutathione (GSH) surface molecular imprinting polymer (SMIP) was successfully prepared by using macroporous adsorption resins (MAR) as substrate, which could separate and purify GSH efficiently.

SMIP was synthesized by chloromethylated modified MAR (LX1180-Cl) as the substrate, N, N’-methylenebisacrylamide (NMBA) as a crosslinker, GSH as a template, acrylamide (AM) and N-vinylpyrrolidone (NVP) as functional monomers. The morphology and structure of the polymer were characterized by scanning electron microscope and Fourier transforms infrared spectroscopy.

The maximum adsorption capacity toward GSH was 39.0 mg/g and the separation decree had relation to L-cysteine (L-cys) was 4.2. The optimal operation conditions were studied in detail and the got as follows: the molar ratios of NMBA, AM, GSH and NVP, were 7.0, 0.8 and 0.5. The optimal time and temperature were 14 h and 40°C, respectively. The Langmuir and pseudo-first-order model were fitting these adsorption characteristics well.

GSH has a diversity of medicinal and bioactive functions, so the purpose of this study representing a method in separate and purify technology of GSH, which provided a way for the development of medicine.

This contribution provided a novel way to separate GSH from L-cys. Under the optimal conditions, the maximum adsorption capacity toward GSH was 39.0 mg/g and the separation decree had relation to L-cys was 4.2.