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In recent decades, advancements in biosensors technology have made fluorescent biosensor pivotal for biomolecular recognition. This paper aims to provide an in-depth analysis of polymerase chain reaction (PCR) fluorescent biosensor detection technology for identifying Escherichia coli (E. coli), setting the stage for future developments in the field.

The review of literature on PCR fluorescent biosensor detection technology for E. coli over the past decades includes discussions on traditional biological fluorescent detection, quantitative PCR fluorescent detection and digital fluorescent detection technology.

Advancements in fluorescent biosensor technology enable precise measurement of fluorescent signals, and when integrated with microfluidic technology, produce compact, reagent-efficient digital sensor devices.

This paper provides a comprehensive review of recent fluorescent detection technology for pathogenic E. coli, assessing method efficiencies and offering insights to advance the field.

As transition countries shift to a mixed welfare system, the accountability of non-government organizations (NGOs) becomes critical to quality services. Yet, poor financial and managerial practices of some NGOs in China have led to distrust from citizens. The purpose of this paper is to use a democratic accountability framework to examine citizen participation in NGOs as an approach to understand an angle of this distrust. Does the Chinese language academic literature about NGO accountability engage with concepts of participation in NGO governance, management and service use?

The method was content analysis of a search of words and concepts relating to NGOs, participation and accountability in the available Chinese language literature on NGO accountability through the newly developed search engine Wenjin Search of the National Library of China.

The analysis found that most Chinese literature only emphasizes problems of accountability, causes and regulatory solutions. When the literature includes participation, it refers to it as a platform for civil society, rather than a process of accountability within an NGO.

Searching by keywords in one search engine may not be exhaustive. The results probably reflect most of the current research of Chinese scholars, considering the depth of the search engine.

Formal NGOs are relatively new in the Chinese political landscape; and government regulations are largely administrative and unenforced. At conceptual and political levels, the absence of discussion about other forms of accountability ignores questions about public dissatisfaction with NGO performance and the public’s willingness to contribute to NGO effectiveness, and civic engagement.

An implication is that until Chinese NGO research also incorporates democratic accountability concepts, it will continue to ignore the internal and external drivers from citizens for NGO change. Transition country NGOs that encourage participation have the potential to engender greater accountability in the organization, community and in state relations.

A green innovation strategy is an important step for enterprises to balance economic and environmental. As the executors of strategic decisions, the attitude and capabilities of senior managers determine the effectiveness of implementing green innovation. Therefore, this paper aims to explore the relationship between executive compensation incentives and green innovation.

Based on the data of heavily polluting enterprises listed in China's A-share market from 2015 to 2020, this study constructs an OLS model with fixed effects of time and industry, and uses the mediation three-step method to verify the correlation between executive compensation incentives, innovation openness and green innovation. Meanwhile, the grouping regression was used to test the moderating effect of environmental regulation on executive compensation incentives.

The empirical results show that executive salary incentives promote green innovation and equity incentives inhibit green innovation; the openness breadth partially mediates the relationship between salary incentives, equity incentives and green innovation, while the openness depth only partially mediates the relationship between equity incentives and green innovation; and environmental regulation positively moderates executive incentives.

Due to sample selection and variable measurement, the study lacks certain generality. Therefore, future research needs to further analyze the internal factors affecting green innovation from multiple dimensions.

This study provides a new evidence for analyzing how executive compensation measures affect green innovation, and further enhances the mediating mechanism of open innovation.

This study has significant theoretical implications for examining the intra-firm factors that affect green innovation.

In this study, a highly sensitive and quantitative analysis method using surface-enhanced Raman scattering (SERS)-labeled immunoassay is adopted for bisphenol A bisphenol A (BPA) detection in water samples.

Primarily, an excellent SERS immuno-nanoprobe is prepared, which relays on Au/Ag core-shell nanoparticles tagged 4-mercaptobenzoic acid (4MBA) and labeled with specific antibody against BPA. Second, the coating antigen of 4,4-Bis(4-hydroxyphenol) valeric acid (BVA) coupling poly-L-lysine (PLL) conjugate (BVA-PLL) is fastened on the substrate. Based on competitive immunoassay, the antibody labeled on SERS immuno-nanoprobe will bind with the free BPA and BVA-PLL competitively.

A calibration curve was obtained by plotting the intensity of SERS signal of 4MBA at 1007 cm⁻¹ versus the concentration of BPA. The results indicated that the limit of detection (LOD) for BPA is 1 ng/mL and present a great capacity for higher sensitivity. Furthermore, the method was able to quantitatively detect BPA in water samples, which was validated by high performance liquid chromatography (HPLC).

The method was developed based on competitive immunoassay, and the conjugate (BVA-PLL) was chosen as the coating antigen. Au/Ag core-shell nanoparticles played as the SERS active substrate and were labeled with Raman reporter. The value of this paper is supplying a wide potential for analysis of target analytes in the environmental monitoring and food safety.

The purpose of this study is to investigate the contingencies of supply chain risk management (SCRM) in manufacturing multinational corporations (MNCs) by exploring the moderating role of international asset dispersion in the performance effect of SCRM, as well as the counteraction effect of supply chain integration (SCI).

Multi-country survey data from a sample of 378 responses collected by the sixth round International Manufacturing Strategy Survey were analyzed. Hierarchical regression analysis was conducted to test the proposed hypotheses.

The results demonstrate that SCRM improves the operational effectiveness of manufacturing MNCs but this performance effect is attenuated by international asset dispersion. Nevertheless, external integration can counteract the negative effect of international asset dispersion and ensure the efficacy of SCRM practices.

The results of this study provide suggestions for supply chain managers of manufacturing MNCs to better conduct SCRM practices under the condition of dispersed international assets. Besides, supply chain managers can leverage external integration to ensure the efficacy of SCRM practices.

Although there are plenty of studies on the relationship between SCRM and operational performance, prior findings are inconsistent. This study sheds light on the SCRM-operational performance relationship by considering how a manufacturing MNC’s international asset dispersion can influence the efficacy of SCRM practices and how SCI can attenuate the negative effect of international asset dispersion.

Through the case analysis of China’s digital library, the purpose of this paper is to make readers understand the development status, achievements and problems of China’s digital library. At the same time, it also enables readers to understand the common application forms of these modern information technologies in digital libraries, so as to provide a reference basis for the application of new technologies in other countries’ digital libraries.

On the basis of literature review, the authors have analyzed a conspicuous collection of related papers in order to make a comprehensive summary and elaboration on the present situation of modern information technology application in Chinese digital libraries.

This paper defines and analyses the concepts, contents and relationships of the three most important modern Big Data information technologies, and then completes the analysis of the current situation of the application of information technology in Chinese digital libraries. The block chain can achieve more accurate information collection, more secure information storage and more effective information dissemination. Artificial intelligence can improve the existing digital library service level in three aspects: resource construction, information organization and information service. Internet plus will help transform the traditional digital library business mode in order to adapt to the change of user-centered service.

This is among the first structured attempts of comprehensively and generally summarizing the application of modern information technology in Chinese digital libraries. It also contains an original exploration of the relationship between the main kinds of modern information technology. Based on this, a relatively complete application scenario and possible vision of modern information technology in digital library can be built.

The present study provides a comprehensive review of the advancements in five active heating modes for cold-proof clothing as of 2021. It aims to evaluate the current state of research for each heating mode and identify their limitations. Further, the study provides insights into the optimization of intelligent temperature control algorithms and design considerations for intelligent cold-proof clothing.

This article presents a classification of active heating systems based on five different heating principles: electric heating system, solar heating system, phase-change material (PCM) heating system, chemical heating system and fluid/air heating system. The systems are analyzed and evaluated in terms of heating principle, research advancement, scientific challenges and application potential in the field of cold-proof clothing.

The rational utilization of active heating modes enhances the thermal efficiency of cold-proof clothing, resulting in enhanced cold-resistance and reduced volume and weight. Despite progress in the development of the five prevalent heating modes, particularly with regard to the improvement and advancement of heating materials, the current integration of heating systems with cold-proof clothing is limited to the torso and limbs, lacking consideration of the thermal physiological requirements of the human body. Additionally, the heating modes of each system tend to be uniform and lack differentiation to meet the varying cold protection needs of various body parts.

The effective application of multiple heating modes helps the human body to maintain a constant body temperature and thermal equilibrium in a cold environment. The research of heating mode is the basis for realizing the temperature control of cold-proof clothing and provides an effective guarantee for the future development of the intelligent algorithms for temperature control of non-uniform heating of body segments.

The integration of multiple heating modes ensures the maintenance of a constant body temperature and thermal balance for the wearer in cold environments. The research of heating modes forms the foundation for the temperature regulation of cold-proof clothing and lays the groundwork for the development of intelligent algorithms for non-uniform heating control of different body segments.

The present article systematically reviews five active heating modes suitable for use in cold-proof clothing and offers guidance for the selection of heating systems in future smart cold-proof clothing. Furthermore, the findings of this research provide a basis for future research on non-uniform heating modes that are aligned with the thermal physiological needs of the human body, thus contributing to the development of cold-proof clothing that is better suited to meet the thermal needs of the human body.