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Gut microbial changes are associated with diseases such as obesity and type 2 diabetes and may be influenced by diet patterns. Thus, this paper aims to investigate the effects of alcohol, sodium chloride (NaCl) and dietary restriction on the composition of the gut microflora.

Five-week-old male C57BL/6N mice were orally administered by gavage with ethanol (ET; 4 g/kg), 200 µL of 6% NaCl (NC), or equivalent volumes of phosphate-buffered saline (ET-control and NC-control) every two days for 10 weeks. The mice were also orally administered by gavage with the recommended diet (RD-control; 3 g per mouse per day), or with 40% (RD-40) or 60% restricted level (RD-60). DNA samples obtained from mice ceca were sequenced using the Illumina MiSeq Sequencing system; raw data were analyzed using BIOiPLUG software.

This study shows that high ethanol and NaCl consumption, and diet restriction can change the composition of the intestinal microflora, especially Akkermansia muciniphila in ET (p = 0.0050) and NT (p = 0.0366) groups. In addition, the ratio of Bacteriodetes/Firmicutes and the diversity of microflora were generally reduced (p = 0.0487-0.4929).

These results raise the possibility of a relationship between diet patterns, change of intestinal microbiome and disease, which must be further evaluated.

In an effort to reduce ambiguity around customs clearance of items deemed pernicious to public morality and present lessons for customs administration, this research aims to explore ways to improve customs clearance for RealDolls. Furthermore, by suggesting specific ideas to improve the process, this research also aims to contribute to the future customs clearance of socially controversial and legally ambiguous items.

The authors have decided to review studies from ethical and legal perspectives to focus on the legislation and its enforcement issues. The literature review is designed to provide insights on how to incorporate legal and ethical reasoning in the customs clearance process for RealDolls. Supplementary interviews were also conducted with criminal lawyers and customs officials to obtain expert knowledge on domestic legislation and customs control against the sex toys industry.

As a result of the study, it is found that a complete ban on RealDoll infringes on individuals' right to pursue happiness guaranteed by the Constitution and in most cases, cannot be implemented without loopholes. Therefore, it is suggested that the import regulation on RealDolls should be changed to the negative list system, which selectively disapproves certain RealDolls based on a list of RealDolls previously denied clearance by either the Supreme Court or the Customs Clearance Screening Committee. In addition, to have sufficient ethical and legal grounds, it is necessary to expand research on RealDolls regulation and actively introduce the process of obtaining social consensus.

There has been little discussion on regulating RealDolls on the customs clearance level; no specific criterion exists except the public morality clause. This study is vital in that it reviews issues of RealDolls customs clearance from legal and ethical perspectives. Doing so also has a practical significance of providing implications for the customs policies regarding items deemed pernicious to public morality.