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This study aims to determine the appropriate irradiation dose for induction of somaclonal variation in mass of unorganized parenchyma cells derived from sainfoin (Onobrychis viciifolia) tissues.

To investigate the feasibility of UV-B irradiation (312 nm), seeds and callus of sainfoin were exposed to five different intensities (20-100 per cent) for evaluating the effectiveness of phytochemical constituents and cellular behaviour.

Although percentage of seed viability reduced in 20 per cent intensity of UV-B irradiation compared with control seeds, the germination speed significantly enhanced from 3.58 to 5.42. The spectrophotometer analysis confirmed that concentrations of chlorophyll (a and b) decreased after UV-B exposure as compared with control callus. Also, carotenoid content showed significant differences among treated calli. Flavonoid and phenolic contents were observed to gradually increase when the non-irradiated callus subjected to 40 and 60 per cent intensities of UV-B irradiation, respectively. Observation on cellular behaviour such as determination of nuclear and cell areas, mitotic index and chromosomal aberrations were also detected to change in different intensities of UV-B irradiation. Fragmented and aneuploidy aberrations were only observed in exposed cells with more than 40 per cent intensity of UV-B irradiation.

In reality, radiocytological evaluation was proven to be essential in deducing the effectiveness of UV-B irradiation to induce somaclonal variation in callus tissue of sainfoin.

The purpose of this paper is to identify the most dominant pigment of pomegranate explants for natural color coatings and detect the presence of phytochemical constituents and comparison of the antioxidant activities.

Extracts of leaf, stem, peel and seed of in vitro and in vivo growth cultures were prepared for phytochemical constituent and antioxidant activity. The supernatant from 95 per cent methanol was mixed with 15 per cent polyvinyl alcohol (PVA) with the ratio of 1:1 to form a coating system.

Although glycosides was not found in this species, tests for tannins and flavonoids were positive in all samples. The IC50 values were also comparable to commercial antioxidant ascorbic acid with 34.92 per cent inhibition. Chlorophyll a and b were detected in stem and leaf using UV-photospectrometer in 420 and 645 nm wavelengths ranges. The effects of heat and salt on the stability of natural dye colorants mixed with polyvinyl alcohol to form a basic coating system were indicated negatively in in vivo and in vitro growth cultures.

The paper shows that further improvement with co-pigmentations may give a notable mixture from pomegranate extraction for the paint materials or nail varnish. It was also indicated that pomegranate contains some compounds such as polyphenolics that can donate electron/hydrogen easily.

Contributing to the literature on movement structure in authoritarian regimes, this analysis focuses on the structure of two Iranian movements. We use a multi-method approach to analyze the organization of the student and women’s movements in Iran between 1997 and 2008. From 1997 to 2004, a reform government opened political opportunities. The period between 2005 and 2008 was characterized by increased repression. The student movement was organized during the first period as a hybrid composed of several networks linked in a federal structure. As the political context changed, the movement became less centralized. Its strategy shifted from one based in alliance with governing reformers to coalition building outside of the regime. In contrast, the women’s movement was organized as a densely linked web of noncentralized campaigns. The women’s movement overcame divisions as political opportunities closed in the mid-2000s and built a grassroots strategy during the latter part of the decade.

This study aims to analyse the impact of outdoor climate, building configuration and locations of public amenities on pedestrians’ walking behaviour and their outdoor thermal comfort. As outdoor climate can rapidly change within minutes, this study maintains that outdoor thermal comfort should be analysed at the pedestrian scale and within a short period to understand people’s walking behaviour and how it may affect urban activities.

This research analyses the thermal stresses experienced by pedestrians and their walking patterns within a sub-hour timeframe in Darling Square, Sydney. The simulation incorporates multiple outdoor climate analyses with agent-based modelling (ABM) simulation; it runs on a parametric modelling platform to predict walking patterns according to the agents’ innate properties, goals and environmental constraints as the weather fluctuates.

This study demonstrates that mapping outdoor thermal comfort within a shorter timescale could reveal emergent walking patterns that inform pedestrians’ preferences of their surrounding environment and their walking behaviour.

The findings could inform planners and stakeholders on how outdoor climate and building configuration affect pedestrian walking patterns for improving outdoor space quality during the design process or post-occupancy evaluation.

This study integrates ABM that incorporates multiple agents’ goals and innate properties and a micro-climate analysis on diverse context geometries under sub-hour periods, compatible with the pedestrian walking speed and distances at the neighbourhood scale.

The purpose of this article is to provide a general review of the health-care needs in Kenya which focuses on the role of community engagement in facilitating access and diminishing barriers to quality care services. Health-care concerns throughout Kenya and the culture of Kenyan’s health-care practices care are considered.

A comprehensive review covered studies of community engagement from 2000 till present. Studies are collected using Google Scholar, PubMed, EBSCOhost and JSTOR and from government and nongovernment agency websites. The approach focuses on why various populations seek health care and how they seek health care, and on some current health-care delivery models.

Suggestions for community engagement, including defining the community, are proposed. A model for improved health-care delivery introduces community health workers (CHWs), mHealth technologies and the use of mobile clinics to engage the community and improve health and quality of care in low-income settings.

The results emphasize the importance of community engagement in building a sustainable health-care delivery model. This model highlights the importance of defining the community, setting goals for the community and integrating CHWs and mobile clinics to improve health status and decrease long-term health-care costs. The implementation of these strategies contributes to an environment that promotes health and wellness for all.

This paper evaluates health-care quality and access issues in Kenya and provides sustainable solutions that are linked to effective community engagement. In addition, this paper adds to the limited number of studies that explore health-care quality and access alongside community engagement in low-income settings.

The purpose of this study is to examine effects of classroom-level and student-level factors on student engagement in the context of a higher education system vertically differentiated into research, applied and comprehensive university types.

The study used a cross-sectional multilevel design to explain student engagement based on class and student variables. Specifically, the study collects data from 656 students and 61 randomly selected teachers at both levels and uses multilevel modeling to explain relationship patterns.

The results show that institutions vary significantly in student engagement scores. In addition, while a significant variation is found at the student and classroom level, the effects of academic achievement, instructional quality, teaching experience and teacher qualifications on student engagement vary across classrooms in institutions. However, the interaction effect of classroom and student-level variables on student engagement remains non-significant.

The main contribution of this lies in the explanation of student engagement using classroom and student level factors in a vertically differentiated higher education system using multilevel modeling. Student engagement varied in classrooms research universities applied and comprehensive universities.

The purpose of the present study is to understand the role of auxin and cytokinin in stimulating the production of pigmented callus in Orthosiphon stamineus and to gain correlation between the callus colours with their antioxidant capacity and bioactive constituents.

In this study, plant tissue culture was used to induce production of callus of various colours from leaf explants of O. stamineus, via manipulation of plant hormones (0-2.0 mg L⁻¹ indole-3-acetic acid [IAA] and Kinetin [Kin]). The coloured callus was subjected to solvent extraction and used for quantification of its carotenoid, chlorophyll, anthocyanin and phenolic contents. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of the extracts was also evaluated, before and after four weeks of storage at −20°C.

The highest mean (per cent) explants that produced roots (93.33 ± 0.05 per cent) were observed when the cultures were supplemented with 2.0 mg L⁻¹ IAA. The colour of the callus changed with time, from green to cream to brown after two and four months of culture, respectively. Optimum production of green callus was achieved with addition of 2.0 mg L⁻¹ Kin plus 1.0-2.0 mg L⁻¹ IAA to the media, while cream callus in 0.5 mg L⁻¹ Kin plus 2.0 mg L⁻¹ IAA and brown callus in 0.5 mg L⁻¹ Kin plus 1.5 mg L⁻¹ IAA. Green callus was found to contain the highest amount of chlorophylls, carotenoid and anthocyanin, while cream callus contained the highest amount of phenolic compounds. The amount of pigments and secondary metabolites in the callus extracts decreased after four weeks of storage, except anthocyanin. The antioxidant potential of the extracts also increased after storage.

The major compounds identified in the methanolic extracts of O. stamineus-coloured callus are chlorophylls, carotenoids, flavonoids and phenolic acids. Future research work should include improvements in the extraction and identification methods which may lead to detection of other compounds that could attribute to the antioxidant capacity, to complement the findings of the current study.

This analysis provides valuable information on the application of IAA and Kinetin (Kin) to manipulate the content of major pigments with medicinal benefits in O. stamineus by using the plant tissue culture system.

A comparative study on antioxidant capacity and bioactive constituents of pigmented callus from O. stamineus leaves is original. To the best of the authors’ knowledge, this is the first attempt of comparative evaluation on antioxidant potential of O. stamineus-coloured callus produced using IAA and Kin.

The aim of the present study is to gain correlation between saffron colours, their antioxidant capacity and the constituents of the secondary metabolites of three different saffron samples commonly available in the Malaysian market.

In this study, saffron samples imported from Iran, Spain and Kashmir were extracted using methanol and the total antioxidant capacity were analysed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging assay. Ultra-high performance liquid chromatography (UHPLC) and electrospray ionization coupled with mass spectrometry (ESI-MS/MS) were then used to identify secondary metabolites present in the saffron crude extracts. The colour variation of the saffron extract was expressed by the CIELAB colorimeter in terms of lightness (L*), greenness (a*), yellowness (b*), chroma (C) and hue angle (H°). Principle component analysis (PCA) was carried out to analyse the similarities and correlation between variables.

This study reveals that antioxidant has a positive correlation with flavanols, especially quercetin and kaempferol. In addition, colour appearance is not necessarily an indicator for the antioxidant level.

The major classes of polyphenolics identified in saffron methanolic crude extract are flavonoids and phenolic acids. Further improvements in the extraction and identification methods may find other compounds that could attribute to the visual factors and antioxidant capacity as a complement for this preliminary study.

This analysis provides comparison basis and valuable information for saffron consumers in Malaysia depending on the consumers’ purpose of whether to have a certain desired colour or health benefits.

A comparative study about saffron product available in Malaysian market is original. To the best of the authors’ knowledge, this is the first attempt of evaluation on a saffron product that has been carried out in Malaysia.

To better understand how corporate communicators and human resources professionals can champion volunteer activities and youth engagement as evidence of corporate social responsibility (CSR) and sustainability commitment, this chapter explores representations of urban youth conservation–environmental empowerment through a textual analysis of three organizations’ websites: Clearwater, the Philadelphia Zoo, and The Nature Conservancy. In addition to identifying common themes across the websites, I compared each program to the Critical Social Theory of Youth Empowerment (CSTYE) framework consisting of six dimensions for maximum success in empowering youth stakeholders (Jennings, Parra-Medina, Hilfinger-Messias, & McLoughlin, 2006). Recommendations are provided for organizations using environmental–conservation programs targeting young people – and other researchers of this phenomenon.