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Article
Publication date: 3 January 2017

Rachna Sehrawat, Paramjit S. Panesar, Tanya L. Swer and Anit Kumar

This paper aims to extract colour from micro-organisms (as a source of natural pigments) using agro-industrial substrates to replace synthetic media by solid state fermentation…

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Abstract

Purpose

This paper aims to extract colour from micro-organisms (as a source of natural pigments) using agro-industrial substrates to replace synthetic media by solid state fermentation. Nature is filled with colours. Due to health and environmental consciousness among people, use of synthetic colour has declined, and so the need to develop colour from cheap and easily available natural sources (plants, animals, micro-organisms and algae) using a cost-effective technique with higher yield and rapid growth. Monascus purpureus colour is a potent source of compounds (Dimerumic acid, Monacolin-k and -aminobutyric acid) having antimutagenic, antimicrobial and antiobesity, which helps in combating diseases.

Design/methodology/approach

Response surface methodology was used to optimise the biopigments extraction from Monascus purpureus using solid state fermentation.

Findings

The best optimised conditions for biopigments production using Monascus purpureus MTCC 369 were pH 5.4 at 32°C for 8 days 9 hours (8.9 days) from sweet potato peel and pea pod powder, 7.8 (w/w) and 3.9 per cent (w/w), respectively, which gave a final yield of 21 CVU/g. The model F-value of 69.18 and high value of adjusted determination coefficient 96.00 per cent implies high level of significance of the fitted model.

Practical implications

Extracted colour can be used in beverages, confectionery and pharmaceutical industries.

Social implications

Colour produced using Monascus purpureus MTCC 369 is a natural source. As consumers are reluctant to use synthetic colour because of the undesirable allergic reactions caused by them, so a biopigment produced is natural colouring compound with wide application in food sector.

Originality/value

Selected sources of carbon and nitrogen were not used earlier by any researcher to extract biopigment from Monascus purpureus MTCC 369.

Details

Pigment & Resin Technology, vol. 46 no. 1
Type: Research Article
ISSN: 0369-9420

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Article
Publication date: 18 September 2019

Sawinder Kaur, Vivek Mumbarkar, Paramjit S. Panesar, Sushma Gurumayum and Prasad Rasane

The purpose of this study is to isolate yellow pigment producing fungal strain and to determine the media requirement for growth and secondary metabolite production.

176

Abstract

Purpose

The purpose of this study is to isolate yellow pigment producing fungal strain and to determine the media requirement for growth and secondary metabolite production.

Design/methodology/approach

Fifteen soil samples were collected and studied for a pigment producing fungal sources. Selection of a fungus was based on pigment produced and further conditions, such as effect of media composition and light wavelength on pigment production and growth parameters were optimised.

Findings

Out of the isolates analysed, Epicoccum nigrum was selected for further study as this strain has the potential for pigment production. Among all the media evaluated, potato dextrose agar (PDA) was found to be the best media for growth and sporulation, whilst sabouraud dextrose agar (SDA) was only 29 per cent as capable as the best medium. The radial growth rate in case of PDA was 3 ± 0.02 mm/day, while in case of SDA, it was only 1.09 mm/day. Whilst starch as a carbon source was found to increase the radial growth to 5.15 ± 0.02 mm/day, sucrose significantly (p < 0.05) influenced the sporulation (224,000 ± 1,550 spores/ml) of Epicoccum nigrum. Amongst the various nitrogen sources analysed, peptone significantly increased (p < 0.05) the radial growth (6.55 ± 0.02 mm/day) as well as sporulation (220,000 ± 2,100 spores/ml). The observations also indicated that E. nigrum is able to sense and differentiate between light in different wavelength ranges and respond differently in growth and sporulation. The light passing through a red colour sheet resulted in better radial growth (8.5 ± 0.02 mm/day) in comparison to unfiltered light (3 ± 0.02 mm/day). Yellow pigment production in terms of hue values was significantly influenced by the presence of dextrose, peptone and darkness.

Research limitations/implications

The isolated strain could be studied for variable conditions and stress factors for optimal production of the pigment. Recovery and purification studies could be carried out at pilot and industrial scale.

Practical implications

The isolation of a strain producing valuable microbial pigment will increase the alternatives of natural food colours and enhance the its commercial applications

Originality/value

This study identifies Epicoccum nigrum as a potential source of microbial pigment and facilitates its growth and production for possible applications in industrial pigment production.

Details

Pigment & Resin Technology, vol. 48 no. 6
Type: Research Article
ISSN: 0369-9420

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Article
Publication date: 9 May 2019

Sawinder Kaur, Paramjit S. Panesar, Sushma Gurumayum, Prasad Rasane and Vikas Kumar

The extraction of bioactive compounds such as pigments from natural sources, using different solvents, is a vital downstream process. The present study aims to investigate the…

158

Abstract

Purpose

The extraction of bioactive compounds such as pigments from natural sources, using different solvents, is a vital downstream process. The present study aims to investigate the effect of different variables, namely, extraction temperature, mass of fermented rice and time on the extraction process of orevactaene and flavanoid pigment from Epicoccum nigrum fermented broken rice.

Design/methodology/approach

Central composite rotatable design under response surface methodology was used for deducing optimized conditions. The pigments were extracted under conditions of extraction temperature (40-70°C), mass of fermented rice (0.5-1.5 g) and time (30-90 min), using water as the extraction media. The experimental data obtained were studied by analysis of variance. Data were fitted to a second-order polynomial equation using multiple regression analysis.

Findings

The optimum conditions generated by the software for aqueous extraction process, i.e. extraction temperature of 55.7°C, 0.79 g of fermented matter and extraction time of 56.6 min, resulted in a pigment yield of 52.7AU/g orevactaene and 77.2 AU/g flavanoid.

Research limitations/implications

The developed polynomial empirical model for the optimal recovery of the orevactaene and flavanoid pigments could be used for further studies in prediction of yield under specified variable conditions.

Practical implications

The response surface methodology helped in optimizng the conditions for the eco-friendly low-cost aqueous extarction process for orevactaene and flavanoid pigments, produced by Epicoccum nigrum during solid state fermentation of broken rice. This optimization can provide the basis for scaling up for industrial extraction process.

Originality/value

This paper focuses on optimizing the extraction conditions to get the maximum yield of orevactaene and flavanoid pigments, using water as the extracting media. No literature is available on the optimization of the extraction process of Epicoccum nigrum pigments, to the best of the authors’ knowledge.

Details

Pigment & Resin Technology, vol. 48 no. 4
Type: Research Article
ISSN: 0369-9420

Keywords

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