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Article
Publication date: 22 June 2012

Helmi Wasoh, Lee Yook Hengb, Fatimah Abu Bakar, Rahman Wagiran, Abu Bakar Salleh, Nor Azah Yusof, Norhisam Misrond and Fatin Hazimah Abdul Rahmane

The purpose of this paper is to describe a capacitive biosensor device consisting of an enzyme electrode and a simple detector which has been developed for histamine measurement.

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Abstract

Purpose

The purpose of this paper is to describe a capacitive biosensor device consisting of an enzyme electrode and a simple detector which has been developed for histamine measurement.

Design/methodology/approach

In this analysis, degradation of histamine through enzymatic reaction produces signal that is monitored using a simple detector equipped with “astable” multivibrator operation circuit (in capacitor‐resistor circuit).

Findings

Different frequency (f) readings have been obtained for glucose, alcohol and histamine in different concentration levels, showing the ability of this simple device system to measure their dielectric constant (k) as formulated by the equation f=(1.44d)/ [kA (R1+2R2)]. The analysis using smaller electrode gap (d) produces higher value of f, indicating that d, is directly proportional to f. For histamine, by using immobilized enzyme electrode, the results show that the change of dielectric properties during the 300‐second reaction period could also be monitored. A linear relationship is obtained between concentration and frequency from 50 to 200 ppm.

Practical implications

Based on this result, an enzyme electrode and “astable” operation circuits have the potential to be used in the development of a simple capacitive biosensor device.

Originality/value

The paper is an outcome of experimental work carried out to observe capacitive sensing behavior using an immobilized enzyme, to measure biological samples, especially histamine.

Details

Sensor Review, vol. 32 no. 3
Type: Research Article
ISSN: 0260-2288

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