Search results
1 – 4 of 4Huey‐Shi Lye, Sue‐Siang The, Ting‐Jin Lim, Rajeev Bhat, Rosma Ahmad, Wan‐Nadiah Wan‐Abdullah and Min‐Tze Liong
This study aims to evaluate the effect of cell immobilization on bioactive property of lactobacilli‐fermented soymilk.
Abstract
Purpose
This study aims to evaluate the effect of cell immobilization on bioactive property of lactobacilli‐fermented soymilk.
Design/methodology/approach
Agrowastes from durian (Durio zibethinus), cempedak (Artocarpus champeden), and mangosteen (Garcinia mangostana) were used as immobilizers for lactobacilli (Lactobacillus acidophilus FTDC 1331, L. acidophilus FTDC 2631, L. acidophilus FTDC 2333, L. acidophilus FTDC 1733, and L. bulgaricus FTCC 0411) in soymilk fermentation. Fermented soymilk was stored at different temperatures (4°C, 25°C and 37°C) for 168 h and sampled for analyses periodically.
Findings
Scanning electron micrographs showed that cells of lactobacilli were immobilized onto the matrix of agrowastes powder. The proteolytic activity was higher in soymilk supplemented with immobilized lactobacilli at 37°C and 25°C compared to that at 4°C. Soymilk fermented by cells immobilized on cempedak rind powder showed higher proteolytic activity (p<0.0001), followed by durian and mangosteen rinds powder (p<0.001). The highest ACE inhibitory activity was also found in soymilk fermented by cells immobilized on cempedak rind powder for all temperatures studied (p<0.0001). In addition, ACE inhibitory activity was higher in soymilk fermented at 37°C, compared to 25°C and 4°C (p<0.0001).
Originality/value
The results in the paper show that cell immobilization enhances the bioactive property of fermented soymilk, in terms of proteolysis and in‐vitro ACE inhibitory activity.
Details
Keywords
Joo‐Ann Ewe, Wan‐Nadiah Wan‐Abdullah, Abdul Karim Alias, Rajeev Bhat and Min‐Tze Liong
The aim of this study is to examine the bioactive properties of lactobacilli‐fermented B‐vitamin soymilk, namely the in‐vitro antihypertensive property and bioconversion of…
Abstract
Purpose
The aim of this study is to examine the bioactive properties of lactobacilli‐fermented B‐vitamin soymilk, namely the in‐vitro antihypertensive property and bioconversion of isoflavone glucosides to aglycones.
Design/methodology/approach
Lactobacillus acidophilus BT 1088, L. fermentum BT 8219, L. acidophilus FTDC 8633 and L. gasseri FTDC 8131 were investigated for their bioactive potential and enhanced bioconversion of isoflavones in soymilk supplemented with individual B‐vitamins at a concentration of 1 mg/L.
Findings
The supplementation of thiamine, riboflavin, niacinamide, calcium pantothenate, biotin and folic acid enhanced the ACE‐inhibitory activity of lactobacilli in soymilk accompanied by a lower IC50 value compared to the control (P<0.05). The β‐glucosidase specific activity of lactobacilli was also enhanced on supplementation of B‐vitamins, leading to increased bioconversion of isoflavones in soymilk. The concentration of genistein was decreased, accompanied by an increased concentration of genistein on fermentation in the presence of thiamine, niacinamide, biotin, calcium pantothenate and folic acid. Additionally, the supplementation of niacinamide, calcium pantothenate, biotin and folic acid also led to lower concentrations of malonyl daidzin, indicating increased hydrolysis of malonyl daidzin to daidzin. Results from the present study indicated that the supplementation of B‐vitamins could enhance the bioactive potential and bioconversion of isoflavones in lactobacilli‐fermented soymilk.
Originality/value
This work has shown that the supplementation of B‐vitamins in lactobacilli fermented soymilk has exerted in vitro ACE‐inhibitory activity and increased the accumulation of bioactive isoflavone aglycones. To the authors' knowledge, this is the first evaluation reporting on such aspects.
Details
Keywords
Chiu‐Yin Kuan, Kay‐Hay Yuen and Min‐Tze Liong
This paper aims to investigate the physical, chemical and physicochemical characteristics of rice husk on alkaline treatment.
Abstract
Purpose
This paper aims to investigate the physical, chemical and physicochemical characteristics of rice husk on alkaline treatment.
Design/methodology/approach
Rice husk (RM) was alkaline treated to produce soluble (SR) and insoluble residues (IR). Each fraction was characterized and soluble fraction was used to produce nanofibres using electrospinning method.
Findings
Alkaline treatment was effective in isolating insoluble dietary fibre rich IR from the other interfering substances such as soluble dietary fibre, protein and lipids that originally present in RM and leached into SR. The problem of silica induced toxicity and indigestibility of rice husk was overcome by alkaline treatment. Alkaline treatment altered the inherent mineral content (calcium, copper, iron, potassium, magnesium, manganese, sodium, phosphorus, zinc) of rice husk, and increased physicochemical properties such as emulsification ability and stability, water holding ability, mineral binding capacity (calcium, copper, iron, zinc), and antioxidant activity. SR had been successfully electrospun into nanofibres with the addition of polyvinyl alcohol.
Originality/value
The paper investigated the characteristic of rice husk (RM and IR) for the development of functional foods, pharmaceutical products and new bioactives delivery system at the expense of reducing environmental liability.
Details
Keywords
Ting‐Jin Lim, Azhar‐Mat Easa, Abdul‐Alias Karim, Rajeev Bhat and Min‐Tze Liong
The aim of this study is to develop a soy‐based cream cheese (SCC) with textural characteristics comparable to that of commercial dairy cream cheese (DCC) via the addition of…
Abstract
Purpose
The aim of this study is to develop a soy‐based cream cheese (SCC) with textural characteristics comparable to that of commercial dairy cream cheese (DCC) via the addition of microbial transglutaminase (MTG), soy protein isolate (SPI) and maltodextrin (MD).
Design/methodology/approach
Response surface methodology (RSM) was employed in this study to determine the effects of MTG, MD and SPI on firmness of SCC.
Findings
The second‐order model generated via RSM was significant with only a 9.76 per cent variation not explained by the model. The coefficient of regression revealed that MTG, MD and SPI showed significant linear effects (P<0.0001) on the firmness of SCC, while MTG and SPI showed significant quadratic effects. The model successfully predicted and developed a SCC model with similar firmness as that of DCC; via the combination of 2.57 per cent (w/w) of MTG, 19.69 per cent (w/w) of SPI and 19.69 per cent (w/w) of MD. Physicochemical analyses revealed that SCC possessed lower fat content, reduced saturated fatty acid and zero trans fat. Further rheological measurements revealed that SCC was more solid‐like at room temperature, but less elastic at refrigerated temperature compared to DCC. SEM and SDS‐PAGE analyses affirmed that the textural changes of SCC were attributed to MTG‐induced cross‐linking.
Originality/value
The research demonstrated that a non‐dairy cream cheese could be developed using soy. In addition, the SCC also contained better nutritional properties compared to its dairy counterpart.
Details