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This paper aims to focus on the aqueous extraction of natural dye from haematoxylum campechianum L. bark for finishing the bio-mordant cotton fabrics producing value-added, environment-friendly textile products, for biomedical applications.

The study focuses on the creation of eco-friendly bio-mordant cotton fabric using gallic acid and gelatin, Al³⁺ and Fe²⁺ salts and metal mordant. The optimal pH for extraction, structural characterization and phytochemical analysis of the extracted dye were estimated using UV-visible spectrophotometer, FTIR and qualitative analysis. Variations in electrolyte concentration and pH medium were also considered. The study also examines build-up properties, colorimetric values and fastness characteristics of the colored fabrics.

All the dyed fabrics exhibit very good to excellent in terms of antimicrobial resistance against S. aureus and C. allbicans.

Pre-mordant cotton fabrics with Fe²⁺ and a combination of metal and bio-mordant show higher antibacterial resistance against P. aerugionsa. Further, bio-mordant and a combination of both mordant exhibit excellent UV protection and antioxidant activity performance compared to that of undyed fabrics.

This work opens up a huge potential for producing healthy bioactive-colored fabrics used in medical textiles and other usages.