Umesh Luthra, Prabhakar Babu, Remya R.R., Angeline Julius, Yogesh Patel, Ramesh Jajula Veera and Ilma Majeed
β-Carotene is the most appropriate and significant precursor of vitamin A. Synthetic carotene supplements have been known to pose a threat to human health, making natural sources…
Abstract
Purpose
β-Carotene is the most appropriate and significant precursor of vitamin A. Synthetic carotene supplements have been known to pose a threat to human health, making natural sources such as the indefensible choice for the production and extraction of carotene.
Design/methodology/approach
This study considers Blakeslea trispora, a filamentous fungus, as a source of production of carotenoids by fermentation and wet and dry mycelium were used to analyse and obtain better extraction results.
Findings
In this study, natural oils such as soy oil and cottonseed oil were incorporated into fermentation media to increase the production of carotene. For the optimization process, Plackett–Burman and one-factor-at-a-time (OVAT) models were identified as being of great value.
Originality/value
OVAT was carried out for corn starch because it plays a major role in the production of carotene and the corn starch at 30 g/L concentration has shown the maximum activity of 3.48 mg/gm. After optimizing process variables, submerged fermentation was eventually carried out under highly controlled media conditions. The resulting product was quantified using UV spectroscopy and extraction of carotene has been observed in the presence of various solvents. Among a range of solvents used, the methylene Di chloride produced-carotene at 86% recovery at a significantly lower temperature of 35°C.
Details
Keywords
Anne Albert-Cromarias and Angeline Pinglot
This study aims to understand stakeholders’ aspiration needs and analyse their role in shaping the articulation and formulation of Grand Challenges (GCs).
Abstract
Purpose
This study aims to understand stakeholders’ aspiration needs and analyse their role in shaping the articulation and formulation of Grand Challenges (GCs).
Design/methodology/approach
Using a comprehensive single case study approach, this paper investigates a GC at a local scale: the preservation and development of the Gergovia Plateau, a well-known French site of natural and historical interest. It analyses rich qualitative data according to Gioia methodology to understand the grounded needs of stakeholders.
Findings
This research deals with the “why side” of a GC when it emerges. First, it identifies four main dimensions regarding stakeholders’ aspiration needs: economic, cultural, environmental and reputational. Second, it highlights the articulation between these four dimensions and analyse their role for a better formulation and implementation of a GC.
Practical implications
This study helps public management practitioners to better understand the very nature of the GC they wish to address and to structure its foundations by including stakeholders’ aspirations.
Originality/value
This case is original regarding GCs literature by shedding a new light on how to deal with socio-environmental issues in a context of natural and cultural heritage.