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Blockchain technology (BCT) has emerged as a powerful tool for enhancing transparency and trust. However, the relationship between the benefits of BCT and agri-food supply chain performance (AFSCperf) remains underexplored. Therefore, the current study investigates the influence of BCT on AFSCperf and sustainability issues.

Through a comprehensive literature review, various benefits of BCT are identified. Subsequently, a research framework is proposed based on data collected from questionnaire surveys and personal visits to professionals in the agri-food industry. The proposed framework is validated using partial least square structural equation modelling (PLS-SEM).

The findings reveal that BCT positively impacts AFSCperf by improving traceability, transparency, food safety and quality, immutability and trust. Additionally, BCT adoption enhances stakeholder collaboration, provides a decentralised network, improves data accessibility and yields a better return on investment, resulting in the overall improvement in AFSCperf and socio-economic sustainability.

This study offers valuable practical insights for practitioners and academicians, establishing empirical links between the benefits of BCT and AFSCperf and providing a deeper understanding of BCT adoption.

Stakeholders, managers, policymakers and technology providers can leverage these findings to optimise the benefits of BCT in enhancing AFSCperf. Moreover, it utilises rigorous theoretical and empirical approaches, drawing on a multidisciplinary perspective encompassing food operations and supply chain literature, public policy, information technology, strategy, organisational theory and sustainability.

In vitro millifluidic cultures with perfusion are essential tools to analyse and understand the interactions between cells, their matrix and multi-cell populations. The purpose of this paper is to focus on the design and development of a 3D-printed template that can be used for fabrication of a clear view poly (dimethyl siloxane) (PDMS) device. The major objective is to obtain a transparent device prototype that allows perfusion culture of two cell types for multiple days that can be imaged using laser scanning confocal microscopy.

The authors used a two-step approach for achieving the final geometric structure at a faster timeline and lower cost. The first part focuses on comparing the fidelity of the printing templates using fused deposition modelling (FDM) and stereolithography (SLA) printers for a range of dimensions. They then show that the complex geometry chip with connection chambers can be printed using low resolution low cost FormLab SLA printer. The final optimized design was then printed using high-resolution Projet 6000 SLA printer to obtain smoother structures.

In this work, the authors have shown that the FormLab SLA printer yields significantly lower error for printing complex design geometries as compared to FDM printer. Result shows that FormLab printer can be used to achieve a minimum dimension of 0.5 mm. They then use the printer to optimize the device dimension for the culture chip which requires several iterations of printing and experimenting. They showed the two-step protocol of printing the optimized template in a high-resolution SLA printer and further fabricating a clear view millifluidic PDMS device that is compatible confocal microscopy imaging. They used this culture chip for perfusion culture of two cell type, and the controlled fluidic exchange between the two chambers led to the formation of neuroglia junction.

One of the major bottlenecks for obtaining complex geometry in mili/microfluidic device by 3D printing is the need of multiple iterations on printing. This makes the tuning of dimension significantly expensive. Another challenge is to obtain a smooth surface of PDMS that leads to a leak proof clear view device compatible for laser based confocal imaging. The combination of two printers plays a crucial role for the rapid prototyping of the imaging device with flow control. The proposed approach lowers the cost for prototyping of in vitro culture chip with complex geometries to improve on biological research demanding multi-chamber fluidic device.