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In recent decades, advancements in biosensors technology have made fluorescent biosensor pivotal for biomolecular recognition. This paper aims to provide an in-depth analysis of polymerase chain reaction (PCR) fluorescent biosensor detection technology for identifying Escherichia coli (E. coli), setting the stage for future developments in the field.

The review of literature on PCR fluorescent biosensor detection technology for E. coli over the past decades includes discussions on traditional biological fluorescent detection, quantitative PCR fluorescent detection and digital fluorescent detection technology.

Advancements in fluorescent biosensor technology enable precise measurement of fluorescent signals, and when integrated with microfluidic technology, produce compact, reagent-efficient digital sensor devices.

This paper provides a comprehensive review of recent fluorescent detection technology for pathogenic E. coli, assessing method efficiencies and offering insights to advance the field.

Field-effect transistor (FET) has excellent electronic properties and inherent signal amplification, and with the development of nanomaterials technology, FET biosensors with nanomaterials as channels play an important role in the field of heavy metal ion detection. This paper aims to review the research progress of silicon nanowire, graphene and carbon nanotube field-effect tube biosensors for heavy metal ion detection, so as to provide technical support and practical experience for the application and promotion of FET.

The article introduces the structure and principle of three kinds of FET with three kinds of nanomaterials, namely, silicon nanowires, graphene and carbon nanotubes, as the channels, and lists examples of the detection of common heavy metal ions by the three kinds of FET sensors in recent years. The article focuses on the advantages and disadvantages of the three sensors, puts forward measures to improve the performance of the FET and looks forward to its future development direction.

Compared with conventional instrumental analytical methods, FETs prepared using nanomaterials as channels have the advantages of fast response speed, high sensitivity and good selectivity, among which the diversified processing methods of graphene, the multi-heavy metal ions detection of silicon nanowires and the very low detection limit and wider detection range of carbon nanotubes have made them one of the most promising detection tools in the field of heavy metal ions detection. Of course, through in-depth analysis, this type of sensor has certain limitations, such as high cost and strict process requirements, which are yet to be solved.

This paper elaborates on the detection principle and classification of field-effect tube, investigates and researches the application status of three kinds of FET biosensors in the detection of common heavy metal ions. By comparing the advantages and disadvantages of each of the three sensors in practical applications, the paper focuses on the feasibility of improvement measures, looks forward to the development trend in the field of heavy metal detection and ultimately promotes the application of field-effect tube development technology to continue to progress, so that its performance continues to improve and the application field is constantly expanding.

The purpose of this paper is to explore the influential mechanism of consumer behaviour and identity towards Neo-Chinese fashion from the perspectives of cultural identity and emotional self-regulation mechanism.

Through empirical methods such as questionnaire surveys and regression analysis, nine hypotheses were proposed and tested based on 253 valid samples.

The results indicate that perceived value has a significant positive impact on pride emotion, anxiety emotion and cultural identity, respectively. Cultural pride (anxiety) emotion also has a significant positive (negative) impact on identity. The study also finds that anxiety emotion plays a significant mediating effect in the relationship between perceived value and cultural identity, which of country-of-origin plays a significant negative moderating role.

This paper reveals the psychological mechanisms and implementation paths through which fashion consumption and Neo-Chinese design drive cultural identity. It provides a theoretical basis for optimizing and innovating strategies to guide the evolution of Neo-Chinese cultural identity in the new context.

Research on corporate social responsibility (CSR) within the real estate sector is limited, and the precise workings of its impact are still unclear. Under the premise that real estate enterprises face environmental uncertainty in China, this study explored the impact of CSR on real estate enterprise value.

To investigate the impact of CSR on enterprise value, we studied 111 real estate enterprises with A-shares listed on Shanghai and Shenzhen stock exchanges from 2010 to 2020, and performed empirical tests to determine the moderating effect of environmental uncertainty on this relationship.

(1) The fulfillment of corporate social responsibility (CSR) significantly influences the value of real estate enterprises. A sub-dimensional analysis reveals that fulfilling stakeholder and social welfare responsibilities within CSR positively impacts enterprise value, whereas environmental responsibility does not exert a notable effect. (2) The uncertainty associated with environmental changes profoundly affects the relationship between CSR and the value of real estate enterprises. More precisely, as environmental uncertainty increases, it amplifies the beneficial impact of CSR on enterprise value.

These findings are valuable for real estate enterprises as they navigate the transition towards sustainable development, and they also provide insight for the government in formulating policies aimed at regulating the real estate sector.

This study complements the existing discussion and research on corporate social responsibility (CSR) and enterprise value in the real estate industry, while elucidating the underlying mechanism of how environmental uncertainty mediates the relationship between the two.

The purpose of this article is to prepare graphene/polyimide composite materials for use as bearing cage materials, improving the friction and wear performance of bearing cages.

The oil absorption and discharge tests were conducted to evaluate the oil content properties of the materials, while the mechanical properties were analyzed through cross-sectional morphology examination. Investigation into the tribological behavior and wear mechanisms encompassed characterization and analysis of wear trace morphology in PPI-based materials. Consequently, the influence of varied graphene nanoplatelets (GN) concentrations on the oil content, mechanical and tribological properties of PPI-based materials was elucidated.

The composites exhibit excellent oil-containing properties due to the increased porosity of PPI-GN composites. The robust formation of covalent bonds between GN and PPI amplifies the adhesive potency of the PPI-GN composites, thereby inducing a substantial enhancement in impact strength. Notably, the PPI-GN composites showed enhanced lubrication properties compared to PPI, which was particularly evident at a GN content of 0.5 Wt.%, as evidenced by the minimization of the average coefficient of friction and the width of the abrasion marks.

This paper includes implications for elucidating the wear mechanism of the polyimide composites under frictional wear conditions and then to guide the optimization of oil content and tribological properties of polyimide bearing cage materials.

In this paper, homogeneously dispersed PPI-GN composites were effectively synthesized by introducing GN into a polyimide matrix through in situ polymerization, and the lubrication mechanism of the PPI composites was compared with that of the PPI-GN composites to illustrate the composites’ superiority.

The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-12-2023-0415

This paper aims to investigate the effect of different wing height layouts on the aerodynamic performance and flow structure of high-speed train, in a train-wing coupling method with multiple tandem wings installed on the train roof.

The improved delayed detached eddy simulation method based on shear stress transport k- ω turbulence model has been used to conduct computational fluid dynamics simulation on the train with three different wing height layouts, at a Reynolds number of 2.8 × 10⁶. The accuracy of the numerical method has been validated by wind tunnel experiments.

The wing height layout has a significant effect on the lift, while its influence on the drag is weak. There are three distinctive vortex structures in the flow field: wingtip vortex, train body vortex and pillar vortex, which are influenced by the variation in wing height layout. The incremental wing layout reduces the mixing and merging between vortexes in the flow field, weakening the vorticity and turbulence intensity. This enhances the pressure difference between the upper and lower surfaces of both the train and wings, thereby increasing the overall lift. Simultaneously, it reduces the slipstream velocity at platform and trackside heights.

This paper contributes to understanding the aerodynamic characteristics and flow structure of a high-speed train coupled with wings. It provides a reference for the design aiming to achieve equivalent weight reduction through aerodynamic lift synergy in trains.

This study aims to prepare thymol-based deep eutectic solvents (DESs) and use them as lubricates for friction and wear tests to simulate the wear conditions of hybrid bearings.

Through the characterization and analysis of the morphology of wear scars and the elemental composition of friction films, the tribological behavior and wear mechanism of sample materials as lubricants were investigated and the anti-wear mechanism of thymol-based DESs was discussed.

The findings demonstrate that because of the formation of a fluid lubrication film and excellent kinematic viscosity, the lubrication effect of the prepared DES is improved by about 50% compared to the base lubricating oil. The prepared [Ch]Cl-thymol DES has a better anti-friction and lubrication effect than citric-thymol, EG-thymol and urea-thymol DESs, with an average friction coefficient of about 0.04.

In this work, the friction reduction properties of thymol-based DESs were studied as lubricants for the first time, and the lubrication mechanism of sample materials was investigated.