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This study aims to enhance the resilience of foamed concrete (FC) against carbonation and water absorption (WA) by integrating microorganisms, specifically Aspergillus iizukae EAN605.

The focus was on understanding how variables such as microorganism concentration, concrete density and water-to-cement (w/c) ratio affect these properties. Optimal results were observed under specific conditions—FC density set at 1800 kg/m³, a w/c ratio of 0.5 and an Aspergillus iizukae EAN605 concentration of 0.5 g/L—resulting in significant reductions in carbonation and WA compared to standard FC.

It is observed that fungi not only fill pores with calcium oxalate but also limit carbonation by consuming CO₂ and block water penetration through their mycelial network. A central composite design within response surface methodology was employed for the experimental design, resulting in mathematical models that align closely with the empirical data. The models identified the most effective parameters for minimizing carbonation depth: FC density at 1970 kg/m³, fungal concentration at 0.585 g/L and w/c ratio at 0.470. Further regression analysis showed a high correlation between the experimental data and the predicted outcomes, with a coefficient of determination (R²) of 92.29 and a model F-value of 16.45.

Statistical analysis highlighted the significant roles of density and fungal concentration in these reductions. Besides, scanning electron microscopy provided visual evidence of fungal-mediated mineral formation in FC, supporting the empirical findings. Overall, the study demonstrated the effective use of Aspergillus iizukae EAN605 in enhancing the durability of FC, marking an innovative stride in sustainable construction materials.