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This study aims to use an additive process for the first time to develop a microfluidic device that uses centrifugal technique for precise and repeatable generation of microdroplets. Droplets have versatile applications in life sciences, but so far centrifugal devices for their production have been made mainly using standard subtractive techniques. This study focused on evaluating the applicability of 3D printing technology in the development of centrifugal microfluidic devices and investigating their properties and future applications.

First, the background of this interdisciplinary research, including the principle of droplet microfluidics and the centrifugal technique, is explained. The developed device has the form of a disc (similar to an audio CD), containing an integrated microfluidic system for droplet generation. The disc is rotated at a specific spin profile to induce controlled liquid flow and accurate production of oil-in-water microdroplets. The device was fabricated using material jetting technology. The design, operation principles, printing process parameters and post-processing steps are explained in detail.

The device was thoroughly characterised, including its mechanical properties, the impact of chemical treatment and the flow measurement of the liquids. The study confirms that the disc can be applied to produce various emulsions using centrifugal force alone. 3D printing technology enables potential mass production and other applications of the device.

The 3D printing process allowed for easy design, fabrication and duplication of the device. Compared to standard PMMA discs, a simpler fabrication protocol and a more flexible and monolithic structure were obtained. The device can be adapted to other microfluidic processes in a lab with high potential for point-of-care applications.