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The purpose of this study is to evaluate the tea stem natural dye was extracted from tea stem waste and applied to dyeing silk fiber, after which the properties of dyed samples were tested and analyzed.

The dyeing process was optimized using the response surface methodology (RSM) approach. Dyeing temperature, pH and time were chosen as variables and the color difference value as a response. The properties of dyed samples were tested and analyzed.

The optimized dyeing process was as follows: dyeing temperature 70°C, pH 3.5 and time 110 min. The K/S and color difference value of silk fiber dyed with the optimal process dye enzymatic oxidation with laccase was 1.4 and 27.8, respectively. The silk fiber dyed has excellent color fastness, antioxidant and antibacterial property, which greatly increases the added value of the dyed products. Furthermore, the optimized dyeing process did not significantly affect the strength properties and handle of the silk fiber.

Researchers have not used statistical analysis to optimize the process of dyeing process of silk fiber by tea stem natural dye enzymatic oxidation with laccase using response surface methodology. Additionally, this dyeing process was a low-temperature dyeing process, which not only saves energy consumption and reduces silk fiber damage but also obtains superbly dyeing results and biological functional properties, achieve the effects of waste utilization and clean dyeing.

In traditional Chinese medicine (TCM), the mechanism of disease (MD) constitutes an essential element of syndrome differentiation and treatment, elucidating the mechanisms underlying the occurrence, progression, alterations and outcomes of diseases. However, there is a dearth of research in the field of intelligent diagnosis concerning the analysis of MD.

In this paper, we propose a supervised Latent Dirichlet Allocation (LDA) topic model, termed MD-LDA, which elucidates the process of MDs identification. We leverage the label information inherent in the data as prior knowledge and incorporate it into the model’s training. Additionally, we devise two parallel parameter estimation algorithms for efficient training. Furthermore, we introduce a benchmark MD identification dataset, named TMD, for training MD-LDA. Finally, we validate the performance of MD-LDA through comprehensive experiments.

The results show that MD-LDA is effective and efficient. Moreover, MD-LDA outperforms the state-of-the-art topic models on perplexity, Kullback–Leibler (KL) and classification performance.

The proposed MD-LDA can be applied for the MD discovery and analysis of TCM clinical diagnosis, so as to improve the interpretability and reliability of intelligent diagnosis and treatment.