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1 – 3 of 3Weiwei Yue, Yuwei Cao, Shuqi Xie, Kang Ning Cheng, Yue Ding, Cong Liu, Yan Jing Ding, Xiaofeng Zhu, Huanqing Liu and Muhammad Shafi
This study aims to improve detection efficiency of fluorescence biosensor or a graphene field-effect transistor biosensor. Graphene field-effect transistor biosensing and…
Abstract
Purpose
This study aims to improve detection efficiency of fluorescence biosensor or a graphene field-effect transistor biosensor. Graphene field-effect transistor biosensing and fluorescent biosensing were integrated and combined with magnetic nanoparticles to construct a multi-sensor integrated microfluidic biochip for detecting single-stranded DNA. Multi-sensor integrated biochip demonstrated higher detection reliability for a single target and could simultaneously detect different targets.
Design/methodology/approach
In this study, the authors integrated graphene field-effect transistor biosensing and fluorescent biosensing, combined with magnetic nanoparticles, to fabricate a multi-sensor integrated microfluidic biochip for the detection of single-stranded deoxyribonucleic acid (DNA). Graphene films synthesized through chemical vapor deposition were transferred onto a glass substrate featuring two indium tin oxide electrodes, thus establishing conductive channels for the graphene field-effect transistor. Using π-π stacking, 1-pyrenebutanoic acid succinimidyl ester was immobilized onto the graphene film to serve as a medium for anchoring the probe aptamer. The fluorophore-labeled target DNA subsequently underwent hybridization with the probe aptamer, thereby forming a fluorescence detection channel.
Findings
This paper presents a novel approach using three channels of light, electricity and magnetism for the detection of single-stranded DNA, accompanied by the design of a microfluidic detection platform integrating biosensor chips. Remarkably, the detection limit achieved is 10 pm, with an impressively low relative standard deviation of 1.007%.
Originality/value
By detecting target DNA, the photo-electro-magnetic multi-sensor graphene field-effect transistor biosensor not only enhances the reliability and efficiency of detection but also exhibits additional advantages such as compact size, affordability, portability and straightforward automation. Real-time display of detection outcomes on the host facilitates a deeper comprehension of biochemical reaction dynamics. Moreover, besides detecting the same target, the sensor can also identify diverse targets, primarily leveraging the penetrative and noninvasive nature of light.
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Keywords
Yaowei Zhang, Tiantian Cao, Siqi Liu and Shuqi Chen
The inconsistent results shown in previous group faultline research have created a need for investigating the underlying mechanisms of the faultline's effects. This study focuses…
Abstract
Purpose
The inconsistent results shown in previous group faultline research have created a need for investigating the underlying mechanisms of the faultline's effects. This study focuses on clarifying the competing mediating roles of information diversity and team conflict in the nonlinear relationship between board faultlines (BF) and decision quality.
Design/methodology/approach
This study is empirically tested with the questionnaire data from 105 Chinese listed companies.
Findings
This study finds: (1) an inverted U-shaped curve relationship between BF and board decision quality and (2) that the joint mediating effect of team conflict and information diversity leads to the inverted U-shaped curve relationship between BF and decision quality. Specifically, BF shows a U-shaped curve relationship with team conflict and an inverted U-shaped curve relationship with information diversity. Either too weak or too strong faultlines will inhibit the positive effects of information diversity and amplify the negative effects of team conflicts, leading to low-quality decisions.
Originality/value
This study contributes to the research on: (1) board governance as it clarifies the effect of BF on the board decision-making process and its quality, which helps to open the black box of board decision-making and (2) group faultlines as it reveals how information diversity and team conflict can play a joint mediating role in the functioning of team faultlines.
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Xingyang Chen, Linlin Ma, Haoping Xie, Fengting Zhao, Yufeng Ye and Lin Zhang
The purpose of this paper is to present a crack initiation mechanism of the external hydrogen effect on type 304 stainless steel, as well as on fatigue crack propagation in the…
Abstract
Purpose
The purpose of this paper is to present a crack initiation mechanism of the external hydrogen effect on type 304 stainless steel, as well as on fatigue crack propagation in the presence of hydrogen gas.
Design/methodology/approach
The effects of external hydrogen on hydrogen-assisted crack initiation in type 304 stainless steel were discussed by performing fatigue crack growth rate and fatigue life tests in 5 MPa argon and hydrogen.
Findings
Hydrogen can reduce the incubation period of fatigue crack initiation of smooth fatigue specimens and greatly promote the fatigue crack growth rate during the subsequent fatigue cycle. During the fatigue cycle, hydrogen invades into matrix through the intrusion and extrusion and segregates at the boundaries of α′ martensite and austenite. As the fatigue cycle increased, hydrogen-induced cracks would initiate along the slip bands. The crack initiation progress would greatly accelerate in the presence of hydrogen.
Originality/value
To the best of the authors’ knowledge, this paper is an original work carried out by the authors on the hydrogen environment embrittlement of type 304 stainless steel. The effects of external hydrogen and argon were compared to provide understanding on the hydrogen-assisted crack initiation behaviors during cycle loading.
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