Interdigitated microelectrode geometry for simple electrical Escherichia coli O157:H7 DNA detection
Abstract
Purpose
The purpose of this study is to fabricate a transducer-based TiO2 interdigitated microelectrodes with various gap sizes. The most stable electrical properties have been selected for Escherichia. coli O157:H7 DNA detection.
Design/methodology/approach
Sol-gel was used to synthesize TiO2 nanoparticles. Lift-off photolithography process was used for fabrication of interdigitated electrodes (IDEs) and dry-state DNA detection was done using a Picoammeter.
Findings
TiO2 nanoparticles IDEs with 16-um gap size is able to detect DNA of E. coli in a dry state.
Originality/value
This paper describes IDEs for dry-state detection of E. coli O157:H7 DNA. The technique presented in this paper ensures the high uniformity of nanoparticle deposition on the finger electrode.
Keywords
Acknowledgements
The author would like to thank all staff members of the Institute of Nano Electronic Engineering at Universiti Malaysia Perlis (UniMAP) for their technical advice and contributions, both directly and indirectly.
Citation
Nadzirah, S. and Hashim, U. (2017), "Interdigitated microelectrode geometry for simple electrical
Publisher
:Emerald Publishing Limited
Copyright © 2017, Emerald Publishing Limited