Antioxidant activity of vitamin E enhanced by cyclodextrin inclusion complex
ISSN: 0007-070X
Article publication date: 29 April 2021
Issue publication date: 2 November 2021
Abstract
Purpose
The study aims to find potential method to enhance the stability and antioxidant activity of vitamins.
Design/methodology/approach
Vitamin E (VE) and β-cyclodextrin (CD) were used to prepare the VE-β-CD inclusion complex via a solution method. The yielded VE-β-CD obtained from optimal experimental conditions was characterized. The mechanism of complex formation and the properties of the complex was investigated by steady-state and time-resolved fluorescence techniques. The antioxidant activity of VE-β-CD was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and compared with pure VE.
Findings
Three experimental factors – reaction temperature (T), molar ratio of VE to β-CD (rm) and inclusion time (t) – were studied for the preparation of VE-β-CD. At experimental conditions of T = 60°C, rm = 1:3 and t = 3 h, a maximum inclusion rate of VE (78.62%) was obtained. The Fourier-transform infrared spectroscopy (FTIR) shows that VE molecule was physically encapsulated by β-CD in the yielded product. Subsequentially, the steady-state fluorescence intensity of VE was found to increase upon encapsulation by β-CD due to the hindrance of fluorescence quenching of VE molecules inside the hydrophobic cavity of β-CD, and an inclusion constant K was determined to equal 3.58 × 103 L/mol from the fluorescence results. The fluorescent observation was confirmed by time-resolved fluorescence experiment as the lifetime of VE increased with an increase in β-CD concentration.
Originality/value
The ability of removing radicals by VE was significantly enhanced after the formation of inclusion complex with β-CD.
Keywords
Acknowledgements
Funding: This study was funded by project of first-rate discipline construction (Traditional Chinese Medicine) in Guizhou Province (grant number GNYL[2017]008), and Project of innovation talent team for development of new drug and novel pharmaceutical technology (grant number [2017]5655).This work was supported by grants from the following projects. PhD early development program of Guizhou University of Traditional Chinese Medicine [2020]46, the high-level innovative talents “hundred levels” talent project of Guizhou province [2015]4030, the First-class Discipline Construction Project in Guizhou Province of China (GNYL (2017)008) and the pharmaceutical new dosage form new technology innovation talent team of Guizhou province (2017)5655. The authors express their sincere gratitude to the foundation.
Citation
Chen, W., Liu, D., Zhou, L., Li, Q. and Wu, D. (2021), "Antioxidant activity of vitamin E enhanced by cyclodextrin inclusion complex", British Food Journal, Vol. 123 No. 12, pp. 3988-3998. https://doi.org/10.1108/BFJ-08-2020-0719
Publisher
:Emerald Publishing Limited
Copyright © 2021, Emerald Publishing Limited