Analysis of microscopic images

Abstract

Purpose

The purpose of this paper is to investigate technologies improving image quality and understanding in life‐science microscopy.

Design/methodology/approach

The new technique of high‐content analysis is described, along with the equipment available from various manufacturers. Advances in fluorescence imaging and confocal microscopy are then addressed. The paper concludes by reporting a powerful 3D visualisation package, and equipment for networked viewing of high‐resolution microscopy images.

Findings

High‐content analysis has developed rapidly in the last four or five years, due largely to improvements in the software interface. Automation and powerful software acquire and manage vast quantities of data, allowing scientists experiment afresh on archived images. Improvements in laser scanning techniques and the emergence of microLED arrays assist microscopy imaging of live cells, whilst techniques giving high‐spectral discrimination improve image understanding.

Originality/value

The paper describes how image‐processing technologies are assisting the work of cell biologists. Stresses the importance of software and hardware design to user uptake, which is relevant for all engineers.

Citation

Connolly, C. (2009), "Analysis of microscopic images", Sensor Review, Vol. 29 No. 1, pp. 20-27. https://doi.org/10.1108/02602280910926724